Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 2. mRNA expression levels of VEGF and MMP‑13 in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Relative mRNA expression levels of VEGF in the medial articular cartilage. (B) Relative mRNA expression levels of MMP‑13 in the medial articular cartilage. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; MMP‑13, matrix metalloproteinase‑13; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Expressing, Standard Deviation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 3. Immunohistochemical analysis of VEGF expression in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Immunohistochemistry staining of VEGF in the articular cartilage of the medial tibial plateau (magnification, x400, scale bar=100 µm). (B) Quantification of VEGF positive cells, based on the results of immunohistochemistry staining. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Immunohistochemical staining, Expressing, Immunohistochemistry, Staining, Standard Deviation

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 5. ELISA analysis of serum VEGF concentration of mice among the Sham, Dmm and Dmm+Th groups (n=8 in each group). The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation

Journal: iScience

Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration

doi: 10.1016/j.isci.2022.105050

Figure Lengend Snippet: iRPE cells have similar phenotype and functions as iPSC-RPE cells (A) Schematic for the transforming process. A cocktail of TF-expressing retroviruses was used to transfect De-iPSC-RPE cells. After seven days, iRPE clone was observed in the culture and picked out for subculturing. (B and C) RPE-specific and EMT-associated markers detected by (B) immunostaining and (C) western blotting after cells were cultured for 8 days. The expression pattern of these markers in iRPE cells is more similar to that in iPSC-RPE cells. Scale bar = 50 μm. (D) Electron micrographs of iPSC-RPE cells, De-iPSC-RPE cells, and iRPE cells demonstrated that quite a few microvilli were on the surface of iRPE and iPSC-RPE cells. Scale bar = 0.5 μm. (E and F) The bound and phagocyted POSs (pointed by arrows) in iPSC-RPE, De-iPSC-RPE, and iRPE cells (E) and quantification of phagocytosis (F) as determined by the number of bound and phagocyted POS per field. Scale bar = 50 μm. Data are mean ± SD, one-way ANOVA and post hoc Bonferroni test, n ≥ 5. (G and H) TER analysis (G) and HRP permeability assay (H) showed that iRPE cells maintained the same epithelial integrity as iPSC-RPE cells. Data are mean ± SD, one-way ANOVA and post hoc Bonferroni test, n = 6. (I) Expression levels of PEDF and VEGF from upper and lower chambers were determined by ELISA. iRPE cells and iPSC-RPE cells demonstrated similar secretion patterns. Data are mean ± SD, one-way ANOVA and post hoc Bonferroni test, n = 3.

Article Snippet: PEDF and VEGF were quantified by PEDF ELISA kit (Elabscience, Wuhan, China) and VEGF ELISA kit (Proteintech).

Techniques: Expressing, Subculturing Assay, Immunostaining, Western Blot, Cell Culture, Permeability, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration

doi: 10.1016/j.isci.2022.105050

Figure Lengend Snippet: BMP7 and FOXF2 are critical regulators of EMT in iRPE cells (A) The higher level of BMP7 secreted by iRPE cells compared with De-iPSC-RPE cells was determined by ELISA. Data are mean ± SD, unpaired two-sided t-tests, n = 4. (B and C) The reduced expression level of FOXF2 in iRPE compared with that in De-iPSC-RPE cells was determined by (B) immunostaining and (C) western blotting. Scale bar = 50 μm. (D) The efficiency of bmp7 knockdown was determined by qRT-PCR; shBmp7-1 was slightly more efficient at reducing the mRNA level of bmp7 than shBmp7-2; therefore, it was selected for subsequent experiments. Data are mean ± SD, one-way ANOVA and post hoc Bonferroni test, n = 4. (E) The shBmp7 construct contained the ZsGreen expression element to indicate successful transfection. Scale bar = 50 μm. (F and G) FLAG-FOXF2 overexpression (ov-FOXF2) in (F) iRPE cells (G) shBmp7-iRPE cells. Scale bar = 50 μm. (H and I) The expression levels of RPE-specific markers and EMT markers were determined by (H) western blotting and (I) quantitative analysis. Data are mean ± SD, one-way ANOVA and post hoc Bonferroni test, n = 3. (J) BMP7 expression levels in shCont-iRPE, shBmp7-iRPE, ov-FOXF2- iRPE, and shBmp7 + ov-FOXF2-iRPE. Data are mean ± SD, one-way ANOVA and post hoc Bonferroni test, n = 4. (K) The immunostaining of RPE-specific markers and EMT markers. Overexpression of FOXF2 exhibited similar effects to knockdown of bmp7 in iRPE cells by downregulating RPE markers and upregulating EMT marker. Scale bar = 50 μm.

Article Snippet: PEDF and VEGF were quantified by PEDF ELISA kit (Elabscience, Wuhan, China) and VEGF ELISA kit (Proteintech).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Immunostaining, Western Blot, Knockdown, Quantitative RT-PCR, Construct, Transfection, Over Expression, Marker

Journal: iScience

Article Title: Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration

doi: 10.1016/j.isci.2022.105050

Figure Lengend Snippet: Four TFs transcriptionally regulate bmp7 , foxf2 , lin7a , pard6b , and ppm1a in a direct or indirect manner (A) ELISA analysis demonstrated that BMP7 levels was reduced in 4TFs−nr2e1-RPE, 4TFs−mitf-a-RPE, 4TFs−c-myc-RPE, and 4TFs−crx-RPE cells compared with that in iRPE cells. Data are mean ± SD, one-way ANOVA and post hoc Bonferroni test, n = 4. (B and C) The levels of FOXF2, LIN7A, PARD6B, and PPM1A were determined by (B) western blotting and (C) quantitative analysis. Data are mean ± SD, one-way ANOVA and post hoc Bonferroni test, n = 3. (D) Generation of FLAG-MITF-A-iRPE, FLAG-CRX-iRPE, FLAG-NR2E1-iRPE, and FLAG-C-MYC-iRPE cells. Scale bar = 50 μm. (E–I) Enriched peaks of CRX binding to the target genes and fold enrichment of CRX immunoprecipitation compared with IgG control, as determined by qRT-PCR. Data are mean ± SD, unpaired two-sided t-tests, n = 3. (J–M) Enriched peaks of MITF-A and NR2E1 binding to the target gene lin7a and fold enrichment of MITF-A and NR2E1 immunoprecipitation compared with IgG control, as determined by qRT-PCR. Data are mean ± SD, unpaired two-sided t-tests, n = 3. (N) Schematic model for the regulation of EMT and MET processes by the four TFs. CRX, MITF-A, NR2E1, and C-MYC directly or indirectly regulated the expression of bmp7 , lin7a , pard6b, ppm1a , and foxf2 to inhibit EMT and promote MET.

Article Snippet: PEDF and VEGF were quantified by PEDF ELISA kit (Elabscience, Wuhan, China) and VEGF ELISA kit (Proteintech).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Binding Assay, Immunoprecipitation, Control, Quantitative RT-PCR, Expressing

Journal: Cell Death & Disease

Article Title: USP12 promotes breast cancer angiogenesis by maintaining midkine stability

doi: 10.1038/s41419-021-04102-y

Figure Lengend Snippet: A The relative expression of VEGF-A and VEGF-C in the supernatant of MDA-MB-231 cells overexpressing USP12 was detected by ELISA. B The relative expression of VEGF-A and VEGF-C in the supernatant of MDA-MB-231 cells with USP12 knockdown was detected by ELISA. C , D Migration of HUVECs treated with supernatant from MDA-MB-231 cells with USP12 overexpression ( C ) and USP12 knockdown ( D ). Representative images are shown in the left panel. The quantitative results are shown in the right panel. E , F . Tube formation assay of HUVECs treated with supernatant from MDA-MB-231 cells with USP12 overexpression ( E ) and USP12 knockdown ( F ). Representative images are shown in the left panel. The quantitative results are shown in the right panel. The experiments were repeated three times. ** p < 0.01, *** p < 0.001; mean ± SD.

Article Snippet: Matrigel matrix (356234, Corning), the human VEGF-A ELISA kit (EKO588-96, Boster) and the human VEGF-C ELISA KIT (EKO539-96, Boster) were also used.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Migration, Over Expression, Tube Formation Assay

Journal: Journal of Cosmetic Dermatology

Article Title: Protective Effects of Exogenous Donkey Oil on Skin Healing Under Incisional Wound Damage

doi: 10.1111/jocd.70550

Figure Lengend Snippet: Effects of different concentrations of DO on the content of VEGF (A) and MMP‐9 (B) in the skin tissue of mice after incision injury. # p < 0.05, ### p < 0.001 vs. BC group; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. NC group.

Article Snippet: The ELISA kits IL‐1α and VEGF were purchased from ProteinTech (Wuhan, China, Cat No: KE10024 and KE10009); IL‐6 from Multi Sciences (Hangzhou, China, Cat No: EK206/3‐96); PGE2 from Lanpaibio (Shanghai, China, Cat No: LP‐M05161); MMP‐9 from CUSABIO (Wuhan, China, Cat No: CSB‐E08007m).

Techniques:

Journal: Stem Cell Research & Therapy

Article Title: Tremella polysaccharide microneedles loaded with magnetic dental pulp stem cell intracellular vesicles used for androgenic alopecia

doi: 10.1186/s13287-025-04219-3

Figure Lengend Snippet: The ability of DPSC-EVs to promote angiogenesis in vitro. ( A ) Scratch experiments were conducted to compare the mobility effects of HUVEC 0–12 h in different treatment groups. Representative microscopic images of HUVEC tube formation at 3 h and 6 h under different treatments. ( B ) Western blot analysis of VEGF after 12 h culture. ( C ) Quantitative analysis of tube formation assay ( n = 3, mean ± SD) ( D ) Quantitative analysis of western blot assay ( n = 3, mean ± SD). ( E ) Quantitative analysis of VEGF in HUVEC supernatant after 12 h culture n = 3, mean ± SD. ( F ) Cell mobility analysis of HUVEC after 12 h culture n = 3, mean ± SD. ns, nonsignificant ( p > 0.05), ⁎ p < 0.05, ⁎⁎ p < 0.01, ⁎⁎⁎ p < 0.001, ⁎⁎⁎⁎ p < 0.0001

Article Snippet: Cell staining buffer, mouse TNF-α (tumor necrosis factor α) ELISA kit, mouse IL-10 (Interleukin-10) ELISA kit and human VEGF-a (Vascular endothelial growth factor A) ELISA kit were purchased from Elabscience (Wuhan, China).

Techniques: In Vitro, Western Blot, Tube Formation Assay

Journal: Stem Cell Research & Therapy

Article Title: Tremella polysaccharide microneedles loaded with magnetic dental pulp stem cell intracellular vesicles used for androgenic alopecia

doi: 10.1186/s13287-025-04219-3

Figure Lengend Snippet: Immunofluorescence analysis of hair follicle regeneration. ( A ) Representative images of fluorescent staining of CD31 + and VEGF-A + cells in hair follicles. ( B ) Representative images of Ki-67 + and SOX9 + fluorescent staining in hair follicles. Fluorescence quantitative analysis of ( C ) Ki-67 + cells ( D ) SOX9 + cells ( E ) CD31 + cells ( F ) VEGF-A + cells. ( G ) Western Blot was used to detect VEGF-A, SOX9 and GAPDH, and ( H - I ) quantitative statistics were performed for gray values. ( J ) Representative images of CD86 + and CD206 + fluorescent staining in skin. Fluorescence quantitative analysis of ( K ) CD86 + cells ( L ) CD206 + cells. ( G ) Western Blot was used to detect CD86, iNOS, Arg-1, CD206 and GAPDH, and ( N ) quantitative statistics were performed for gray values. culture n = 3, mean ± SD. ns, nonsignificant ( p > 0.05), ⁎ p < 0.05, ⁎⁎ p < 0.01, ⁎⁎⁎ p < 0.001,⁎⁎⁎⁎ p < 0.0001

Article Snippet: Cell staining buffer, mouse TNF-α (tumor necrosis factor α) ELISA kit, mouse IL-10 (Interleukin-10) ELISA kit and human VEGF-a (Vascular endothelial growth factor A) ELISA kit were purchased from Elabscience (Wuhan, China).

Techniques: Immunofluorescence, Staining, Fluorescence, Western Blot